Home > Sandwich ELISA Kits > Human 3',5'-Bisphosphate Nucleotidase 1 (BPNT1) ELISA Kit

Product Name:

Human 3',5'-Bisphosphate Nucleotidase 1 (BPNT1) ELISA Kit

Cat.#:

JL23260

Regulatory Status:

RUO

Alternative:

PIP; BPntase; Bisphosphate 3-Prime-Nucleotidase; Bisphosphate 3'-nucleotidase 1; PAP-inositol 1;4-phosphatase

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.312-20ng/mL

Sensitivity:

0.164ng/mL

Specificity:

This kit recognizes Human BPNT1 in samples. No significant cross-reactivity or interference between Human BPNT1 and analogues was observed.

Sample Type:

Tissue homogenates;cell lysates and other biological fluids

Assay Procedure:

Research Area:

Signal Transduction

Size & Price:

Human A1BG Polyclonal Antibody

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Product Datasheets

Instruction:

Data
Citations
Sequence / 3D

| BPNT1

3',5'-Bisphosphate Nucleotidase 1(BPNT1)also called bisphosphate 3-prime-nucleotidase, or BPntase, is a member of a magnesium-dependent phosphomonoesterase family. Lithium, a major drug used to treat manic depression, acts as an uncompetitive inhibitor of BPntase. The predicted human protein is 92% identical to mouse BPntase. BPntase's physiologic role in nucleotide metabolism may be regulated by inositol signaling pathways.

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

Concentration (ng/mL)	20	10	5	2.5	1.25	0.625	0.312	0
OD	2.3	1.87	1.25	0.82	0.55	0.41	0.32	0.09
Corrected OD	2.21	1.78	1.16	0.73	0.46	0.32	0.23	-

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Human BPNT1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Sample Type	Range(%)	Average Recovery(%)
Tissue Homogenates(n=8)	84-101	96
Cell Lysates(n=8)	96-108	104

| Linearity

Samples were spiked with high concentrations of Human BPNT1 and diluted with Universal Diluent to produce samples with values within the range of the assay.

		Tissue Homogenates(n=4)	Cell Lysates(n=4)
1 : 2	Range (%)	85-95	90-110
1 : 2	Average (%)	91	96
1 : 4	Range (%)	89-103	105-115
1 : 4	Average (%)	94	108